Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: List of primers used in qRT-PCR.

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Sequencing

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Effects of LZD on levels of TLR-2 (a) and MyD88 (b) mRNA expression during prevention of IND‐induced duodenal ulceration. Levels of TLR-2 and MyD88 mRNA were determined by qRT-PCR assay. β -Actin mRNA was used as internal control for equal loading. Results are expressed as mean ± SEM, n = 3. Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Expressing, Quantitative RT-PCR, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Effects of LZD on levels of TLR-2 and MyD88 protein expression during prevention of IND induced duodenal ulceration. (a) Representative immunoblots for TLR-2 and MyD88 proteins as measured by western blot analysis using specific antibodies. Loading control was monitored by β -actin immunoblotting. Quantitative analysis derived from densitometric scans of western blots of TLR-2 (b) and MyD88 (c) proteins of duodenal mucosa. Results are expressed as mean ± SEM, n = 3. Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Expressing, Western Blot, Derivative Assay, Negative Control

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antiulcerogenic Activity of Li-Zhong Decoction on Duodenal Ulcers Induced by Indomethacin in Rats: Involvement of TLR-2/MyD88 Signaling Pathway

doi: 10.1155/2020/6538156

Figure Lengend Snippet: Localization of TLR-2 and MyD88 in duodenal tissues after IND administration and effect of LZD thereon. (a) Representative pictures showing the immunohistochemical analysis of TLR-2 and MyD88 in sections of duodenal mucosa obtained from rats in normal control, negative control, ESO, and LZD groups. The black arrows denote the immunopositive expression parts of TLR-2 and MyD88. Magnification 200x. (b, c) Quantitative analysis of the immunohistochemical signals as measured by IOD values. Results are expressed as the mean ± SEM ( n = 3). Asterisks and pound signs indicate significant differences: ∗∗ P < 0.01 versus normal control (NC); ## P < 0.01 versus negative control (IND).

Article Snippet: The sections were incubated with rabbit anti-TLR2 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) or rabbit anti-MyD88 antibody (1 : 100 dilution; Bioss antibodies, Beijing, China) overnight at 4°C and then with HRP-conjugated goat anti-rabbit secondary antibody (1 : 100 dilution; Bioss antibodies, Beijing, China).

Techniques: Immunohistochemical staining, Negative Control, Expressing

Journal: Molecular medicine reports

Article Title: Possible role of miR-204 in optic nerve injury through the regulation of GAP-43.

doi: 10.3892/mmr.2017.8341

Figure Lengend Snippet: Figure 1. Expression levels of miR‑204, GAP‑43, TLR4, MyD88 and NF‑κB. Relative levels of miR‑204, GAP‑43, TLR4, MyD88 and NF‑κB in the retina of each group demonstrated by (A) reverse transcription‑quantitative polymerase chain reaction and (B) western blot analyses. (C) Quantification of protein levels. Compared with the normal SD rats, the level of miR‑204 in SD rats with optic nerve injury was significantly increased (**P<0.05, ***P<0.01) and the mRNA level of GAP‑43 was significantly inhibited by optic nerve injury (**P<0.05, ***P<0.01). miR‑204 increased the gene levels of TLR4/MyD88/NF‑κB, and decreased the mRNA and protein expression of GAP‑43. miR, microRNA; GAP‑43, growth‑associated protein-43; TLR4, toll‑like receptor 4; MyD88, myeloid differentiation factor 88; NF‑κB, nuclear factor‑κB; SD, Sprague‑Dawley.

Article Snippet: Primary antibodies against GAP-43, TLR4, MyD88 and NF-κB were from Abcam (Cambridge, UK); the ChemiDocTM XRS gel imaging system and fluorescence qPCR instrument were from Bio-Rad Laboratories, Inc. (Hercules, CA, USA); SYBR-Green qPCR SuperMix was from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA); the TUNEL apoptosis kit was from Beyotime Institute of Biotechnology (Haimen, China); the mini double vertical electrophoresis and mini transfer electrophoresis apparatus were from Beijing 61 Instrument Factory (Beijing, China); the inverted fluorescence microscope was from Leica Microsystems, Inc. (Buffalo Grove, IL, USA).

Techniques: Expressing, Polymerase Chain Reaction, Western Blot

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 8. Effects of TFE from Folium isatidis on the genes expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d) and p65 (e) in lungs tissues of LPS induced ALI mice ( # compared with the control, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing, Control

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 9. Effects of TFE from Folium isatidis on the proteins expression of TLR4 (a), TRAF6 (b), TRIF (c), I 𝜅B (d), p-I 𝜅B (e), p65 (f) and p-p65 (g) in lungs tissues of LPS induced ALI mice ( # compared with the cntrol, ∗ compared with LPS, ∗ P < 0.05, ∗ ∗ / ## P < 0.01).

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques: Expressing

Journal: Pharmacological Research - Modern Chinese Medicine

Article Title: The pharmaceutical applications of total flavonoids extract from Isatis tinctoria L. leaves

doi: 10.1016/j.prmcm.2022.100122

Figure Lengend Snippet: Fig. 10. Regulation of TFE from Folium isatidis on TLR4-TRIF-NF- 𝜅B signaling pathway in LPS induced ALI mice.

Article Snippet: Antibodies against TLR4, RAF6, TRIF, NF- κB p65, p-NF- κB p65, I κB α, p-I κB α and β-actin were urchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA), and econdary antibodies were obtained from Wuhan Boster Biological Techology., Ltd (Wuhan, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Immunomodulation via MyD88-NFκB Signaling Pathway from Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury

doi: 10.3390/ijms23105295

Figure Lengend Snippet: Western blot analysis for MyD88 and phospho-NFκB in the lung tissue at 48 h after LPS-induced ALI. As shown with boxplots, expressions of MyD88 and phospho-NFκB were decreased significantly in the MSC group. GAPDH was used as the loading control. n = 4 mice/group.

Article Snippet: For immunohistochemical analysis, the lung tissue sections from deparaffinized specimens were stained with anti-MyD88 antibody (Gene Tex, GTX112987) or anti-phospho-NFκB p65 antibody (Gene Tex, GTX50254).

Techniques: Western Blot, Control

Journal: International Journal of Molecular Sciences

Article Title: Immunomodulation via MyD88-NFκB Signaling Pathway from Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury

doi: 10.3390/ijms23105295

Figure Lengend Snippet: Immunohistochemical analysis for MyD88 and phospho-NFκB in the lung tissue at 48 h after LPS-induced ALI (×200). ( A ) Compared to the control group, the percentage of MyD88-positive inflammatory cells in the lung tissue as well as the intensity of staining for MyD88 within the inflammatory cells was decreased in the MSC group. ( B ) Consistently, the expression of phospho-NFκB was lower in the MSC group.

Article Snippet: For immunohistochemical analysis, the lung tissue sections from deparaffinized specimens were stained with anti-MyD88 antibody (Gene Tex, GTX112987) or anti-phospho-NFκB p65 antibody (Gene Tex, GTX50254).

Techniques: Immunohistochemical staining, Control, Staining, Expressing

Journal: The Journal of Experimental Medicine

Article Title: Eosinophil-derived neurotoxin acts as an alarmin to activate the TLR2–MyD88 signal pathway in dendritic cells and enhances Th2 immune responses

doi: 10.1084/jem.20062027

Figure Lengend Snippet: EDN-induced IL-6 production was dependent on MyD88. (A) Human monocyte–derived DCs were transfected with or without (Chariot II alone) gripNAhMyD88. After 24 h of culture, the DCs (4 × 10 5 cells/ml) were incubated with EDN at the indicated concentrations for 40 h, and the concentration of IL-6 in the culture supernatant was measured by ELISA. Shown are the data of one experiment representative of two. (B) DCs generated from the bone marrow progenitors of WT (MyD88 +/+ ) and MyD88 knockout (MyD88 −/− ) mice were incubated at 10 6 /ml in the presence of 1 μg/ml Pam3, EDN, or LPS for 48 h, and the production of IL-6 in the culture supernatants was measured by ELISA. The results of one experiment representative of two are presented as the average (mean ± SD) of triplicate wells.

Article Snippet: For the transient silencing of DC MyD88, monocyte-derived iDCs were transduced without or with gripNA MyD88 (5′-CCTGCAGCCAYDDCGGGC-3′; Active Motif) at 10 nmol/10 6 cells using Chariot II (Active Motif) according to the manufacturer's instructions.

Techniques: Derivative Assay, Transfection, Incubation, Concentration Assay, Enzyme-linked Immunosorbent Assay, Generated, Knock-Out